Chlorophyll is an important indicator of plant photosynthesis and the physiological response of plants. The synthesis of chlorophyll content and how much affect not only the relevant trace elements, but also the condition of the plant's habitat conditions. In some cases, the determination of chlorophyll content in plant leaves by chlorophyll meter to a certain extent reflects the photosynthetic physiology of the plant.

The SPAD-502 Chlorophyll Meter measures the relative chlorophyll content per unit leaf area per unit area of ​​the leaf in seconds, ie, the pad value. At present, the chlorophyll meter has been widely used in agricultural production such as garden trees, corn, potato, rice, and wheat, but the accuracy of models for predicting chlorophyll content in different species using padd values ​​still differs.

Chlorophyll content and chlorophyll spad value of Leymus chinensis varied from individual to individual Leymus chinensis in the same range. The sap value was 21.850-46.150 and the coefficient of variation was 20.457%-36.921%. The contents of total chlorophyll in the leaves of the two steppe L. chinensis were the highest, followed by the content of chlorophyll a, and the content of chlorophyll b was the smallest.

Different geography and climatic conditions between the grasslands and the same micro-environment have different soil conditions. There are certain differences in chlorophyll content among different individuals. This is the response of plants to local environmental conditions. Chlorophyll meter measured chlorophyll spad value and chlorophyll content has a significant correlation, spad value as an indirect index of chlorophyll content can reflect the situation of the plant chlorophyll content.

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