In seed DNA extraction experiments, there is a very important tissue grinding instrument, which is a high-throughput tissue grinder. The application of this instrument is very extensive. In recent years, with the in-depth development and frequent development of seed quality testing, the application frequency of high-throughput tissue grinders in seed purity test items is also very high, greatly improving the The work efficiency has increased the extraction efficiency of seed DNA and is therefore very popular with seed inspection industry personnel. The following is a brief introduction to the working principle and precautions of this instrument by the agricultural instrument network Xiao Bian.

High-throughput tissue grinder

1、Working principle of high flux tissue grinder

The high-flux tissue grinder uses vertical oscillation and the oscillating system reciprocates at a high frequency to collide the frozen sample in the grinding tube with the grinding beads. The resulting grinding shear force and impact force completely break the tissue in seconds. Sample grinding, crushing, mixing and cell disruption can be easily achieved in minutes, resulting in a perfect grinding effect.

2. Precautions for high-flux tissue grinders

There are five main points to note about high-throughput tissue grinders. First, the instrument should be placed in a dry and ventilated environment. Second, the sample should be symmetrically distributed in the sample holder to ensure the balance of the sample holder. Thirdly, before closing the safety door, it is necessary to confirm that the fixture has been completely fixed and there is no looseness; When setting the oscillation frequency, it must not exceed the maximum range of 1800 times/minute. Fifth, when freezing the sample, care should be taken to prevent liquid nitrogen from splashing out and causing frostbite.

The above is the working principle and precautions of the high-throughput tissue grinder. At present, the use of high-flux tissue grinders provides important support for the preparation of multiple samples in the test, which not only saves time, but also improves efficiency. The next experiment provides a reliable basis for analysis. This instrument is increasingly becoming an indispensable instrument in the laboratory. Understanding the above working principles and precautions can help operators better understand, use, and manage the instrument, and give the instrument the best grinding results and improve work efficiency.

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